FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861929
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAACTTCAGGGAGA24120.2798374344058501No Hit
GTATTATTGTCAGCACTATGGTAGCCCACTTCCGGAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA21460.24897642381217014No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18950.21985569577076536No Hit
GTGTATTACTGTGCGAGAGTTGTAGAAGTGGGTGGCAATGACTTCTGGGGCCAGGGAGCCCTGGTCATCGTCTC18140.21045816998847933No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA17090.19827619212255304No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16720.19398349516027422No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT14460.16776323803932808No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA13490.15650941086794853No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG13080.15175263855839632No Hit
GTCTCTGGCTACTTCATCAGCAGTGGTTACTTCTGGGGCTGGATCCGGCA13020.15105652553748627No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13000.15082448786384955No Hit
CAATAATACACTGCAAAATCTTCAGACTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12640.14664780973838915No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC12620.14641577206475243No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGTCTGAAGATT12320.14293520696020207No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12260.14223909393929196No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11600.13458185070928116No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11400.13226147397291424No Hit
ATGGTAGCCCACTTCCGGAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT11070.12843285235790883No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11000.12762072050018042No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG10790.12518432492699516No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAACTTCAGGGAGAACTGGTTCTTGGACGTGTCTA10510.12193579749608147No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTAGCAGCGGCTACTTAGCCTGGTACC10360.12019551494380629No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGCAG10290.11938338308607786No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT10250.11891930773880448No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10020.11625087449198253No Hit
CTTCTACAACTCTCGCACAGTAATACACGGCCGTGTCTGCGGCGGTCACA9900.11485864845016237No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9890.11474262961334403No Hit
CATCTATGGTGCATCCAACAGGGCCCCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA9820.1139304977556156No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGACCTCCTGTG9640.11184215869288537No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT9470.1098698384669735No Hit
GGTCTGGAGTGGATTGGGACTATCTTTCATACTGGGAAAACCGACTACAA8930.10360482127878283No Hit
GTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTC8930.10360482127878283No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8890.10314074593150944No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACAC8790.10198055756332597No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)