FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861929
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAACTTCAGGGAGA21530.24978855566989855No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19470.22588867528531933No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19300.22391635505940743No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA15850.18388985635707814No Hit
GTGTATTACTGTGCGAGAGTTGTAGAAGTGGGTGGCAATGACTTCTGGGGCCAGGGAGCCCTGGTCATCGTCTC15260.17704474498479575No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14170.16439869177159602No Hit
CAATAATACACTGCAAAATCTTCAGACTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12430.14421141416520386No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12210.14165899975520027No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12210.14165899975520027No Hit
GTATTATTGTCAGCACTATGGTAGCCCACTTCCGGAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA12180.14131094324474522No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA11720.1359740767511013No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11710.13585805791428296No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10960.12715664515290703No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC10820.12553238143745019No Hit
GTCTCTGGCTACTTCATCAGCAGTGGTTACTTCTGGGGCTGGATCCGGCA10770.12495228725335845No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC10460.12135570331198973No Hit
GTCCCTCACCTGCGCTGTCTCTGGCTACTTCATCAGCAGTGGTTACTTCT10330.11984745843335123No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10300.1194994019228962No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGTCTGAAGATT9700.11253827171379546No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT9570.11103002683515695No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAACTTCAGGGAGAACTGGTTCTTGGACGTGTCTA9400.10905770660924509No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9390.10894168777242673No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9330.10824557475151665No Hit
CTTCTACAACTCTCGCACAGTAATACACGGCCGTGTCTGCGGCGGTCACA9150.10615723568878643No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC9050.10499704732060298No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT8720.10116842570559756No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGACCTCCTGTG8710.1010524068687792No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA200.00709015452.2382954
GTGGTAT4200.051.6854021
TGGTATC4250.050.2432142
GGTATCA12600.043.2079051
GTATCAA33500.040.21691
GCATAAC350.0013316839.52416241
TATCAAC37150.036.2487142
TACTGAT10250.034.6748570
ATCAACG39400.034.0754173
TCAACGC39900.033.44674
CAACGCA40750.032.988855
AACGCAG41600.032.557076
ATACCAT553.3264188E-431.249986
ACGCAGA44150.030.6766517
CGGCCTA450.004699528730.5626315
CGCAGAG44550.030.5555388
AGAGTAC45100.030.25912711
GCAGAGT45900.029.5070639
CAGAGTA46300.029.4006310
CGAACCT755.7891215E-527.85387253
Produced by FastQC (version 0.11.5)