FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383697
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27860.726093766696117No Hit
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA24980.6510345402752693No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19060.4967461304101935No Hit
ATATTATAGTACTCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT18470.48136941388647814No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16900.44045171059455773No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16120.4201231701055781No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16020.41751694696596536No Hit
CTCCTAAGCTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT15730.40995889986108824No Hit
GTCCCTGACCGCTTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC14890.38806662548834103No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14580.37998733375554145No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC14320.3732111535925483No Hit
TTATAGTACTCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT14290.37242928665066444No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13680.3565313254990266No Hit
CACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC13630.35522821392922016No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT13590.3541857246733751No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12820.33411780649835676No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12250.319262334602564No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12020.31326802138145465No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11380.29658819328793296No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG10380.2705259618918052No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10290.2681803610661538No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10250.2671378718103086No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC9970.2598404470193929No Hit
GTCCCAGACCCGCTGCCACTGAAGCGGTCAGGGACCCCGGATTCCCGGGT9730.2535855114843223No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA9720.253324889170361No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9700.2528036445424384No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9700.2528036445424384No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGTATGTTTTATA9630.2509792883447095No Hit
CTTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCACCAGCC9500.24759119826321288No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9460.24654870900736778No Hit
GGCCAAAAGTGTGAGGAGTACTATAATATTGCTGACAGTAATAAACTGCC9400.24498497512360015No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9240.2408150181002197No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9150.23846941727456822No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGTAGCTTAGGAGGCTGCCCTGGTTTCTGCTGGTA8930.23273572636742013No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA8770.22856576934403972No Hit
TCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAAGCGGTCAGGGACC8760.22830514703007843No Hit
GCTTAGGAGGCTGCCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACATA8750.22804452471611716No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8630.22491705694858183No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8390.21866212141351116No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8280.21579527595993717No Hit
TCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATAT8140.21214656356447928No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7930.20667349497129245No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7780.20276416026187327No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7720.20120042637810565No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7660.199636692494338No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7560.1970304693547252No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7220.1881693106800418No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7100.18504184291250647No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGTATGTTTTATACAGCTCCAA6950.18113250820308732No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACT6890.17956877431931967No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC6860.17878690737743586No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6790.1769625511797069No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCTCACACTTTTGGCCAGGGGACCAAG6750.17592006192386178No Hit
GTCTGGGACAGATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATG6730.17539881729593923No Hit
ACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGCTTCAGTGGCAGCGGGTCTGGGACAGATTT6670.17383508341217158No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC6480.16888325944690732No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6390.16653765862125583No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA6310.16445268010956562No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGTATGTTTTATACAGCTCCAACAATAAGAACTA6300.16419205779560436No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6290.16393143548164305No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6240.16262832391183668No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6140.1600221007722239No Hit
ATAGTACTCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA6060.1579371222605337No Hit
GCAGAAACCAGGGCAGCCTCCTAAGCTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGCTT5980.15585214374884349No Hit
GTAAATGAGTAGCTTAGGAGGCTGCCCTGGTTTCTGCTGGTACCAAGCTA5920.15428840986507583No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA5920.15428840986507583No Hit
ATTATAGTACTCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA5720.1490759635858503No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC5710.14881534127188903No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT5630.1467303627601988No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT5560.14490600656246988No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA5560.14490600656246988No Hit
GTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAAGCGGTC5520.14386351730662475No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT5490.14308165036474094No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC5490.14308165036474094No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAAGCGGTCAGGGACCCCG5490.14308165036474094No Hit
ACCCGGGAATCCGGGGTCCCTGACCGCTTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCACCAGC5460.1422997834228571No Hit
CCACTGAAGCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAAT5440.14177853879493454No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC5330.1389116933413605No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC5300.13812982639947669No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5300.13812982639947669No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG5220.13604484788778648No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGGCAGCCTCCTAAGCTACTCATT5200.1355236032598639No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5190.1352629809459026No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC5190.1352629809459026No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC5170.1347417363179801No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC5120.1334386247481737No Hit
GGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAAG5070.1321355131783673No Hit
GGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAAGCGGT4960.12926866772479326No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT4960.12926866772479326No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT4920.12822617846894815No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA4910.1279655561549869No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA4880.12718368921310305No Hit
GTATAAAACATACTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA4880.12718368921310305No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT4830.1258805776432967No Hit
GAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT4750.12379559913160644No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA4560.11884377516634219No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT4540.11832253053841964No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG4470.11649817434069071No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC4460.11623755202672943No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC4450.11597692971276816No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA4390.11441319582900049No Hit
TTATTACTGTCAGCAATATTATAGTACTCCTCACACTTTTGGCCAGGGGA4340.1131100842591941No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT4340.1131100842591941No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTGAGGAGTA4250.11076448343354262No Hit
GCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTGAGG4230.11024323880562006No Hit
GTACCAGCAGAAACCAGGGCAGCCTCCTAAGCTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGA4220.10998261649165879No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG4220.10998261649165879No Hit
GCTTGGTCCCCTGGCCAAAAGTGTGAGGAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGC4110.10711577103808473No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT4090.10659452641016219No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGC4060.10581265946827836No Hit
AACCAGGGCAGCCTCCTAAGCTACTCATTTACTGGGCATCTACCCGGGAA4060.10581265946827836No Hit
CATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGCTTCAGTGGCAGCGGGTCTGGGACAGATTTCA4050.10555203715431707No Hit
TTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGT4030.10503079252639452No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA4030.10503079252639452No Hit
ACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAA3960.10320643632866558No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGGCAGCCTCCTAAGCTACTCATTTACTGGGCATCTACCCGGGAATC3960.10320643632866558No Hit
GGTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAA3920.10216394707282049No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA3910.10190332475885922No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCAGGGCAGCCTCCTAAGCTAC3910.10190332475885922No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC3890.10138208013093664No Hit
AAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT3860.10060021318905282No Hit
GAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTG3850.10033959087509155No Hit
CCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG3840.10007896856113027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC1200.060.161962
GTGGTAT1300.055.5703931
GGAATGC200.00748195251.52703519
GGTATCA3650.044.2968141
GTATCAA10150.043.721111
CGGGATA702.275192E-839.24333617
TATCAAC11750.038.0355262
ATCAACG12300.036.0317343
TCGAGAA601.5025373E-435.7698369
TCAACGC12450.035.569774
AACGCAG12850.035.2548076
CAACGCA12800.034.8608135
ACGGGAT1003.9835868E-1034.3379216
TACGGGT400.00265818334.32896815
CAGCGCT601.38662E-534.3289644
CGCAGAG13750.033.196828
CTACTCA13900.033.04102770
GCAGAGT14150.032.2583929
ACGCAGA13900.032.097867
AGAGTAC14050.031.75517811
Produced by FastQC (version 0.11.5)