FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600755
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18300.3046166906642475No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAG200.0074541851.57908631
CCGACGC200.00749365251.5102428
GTATCAA31800.041.9133031
GGTATCA12350.039.4974141
TATCAAC35800.037.0074582
GTGGTAT4450.035.5095441
ATCAACG37350.035.2555473
TCAACGC37650.034.857414
CAACGCA38500.034.1711435
AACGCAG39750.033.268966
CGCAGAG41400.031.7775148
ACGCAGA41700.031.6310587
AGAGTAC43200.030.77067811
CTATATG450.004719398530.5347751
CAGAGTA43500.030.40094610
TACGGGC4750.030.30073515
TGGTATC5100.030.284982
GCAGAGT43700.030.2618129
GTCGTAA953.3007018E-728.94942530
GGTCGTA1103.8071448E-828.11056529
Produced by FastQC (version 0.11.5)