FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847317
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8680.10244099905938392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38700.040.1959571
GTGGTAT5750.040.013091
GGTATCA15850.038.1310271
TATCAAC44400.035.1924252
TCAACGC46600.033.6783684
ATCAACG46700.033.606253
CAACGCA47700.032.757735
AACGCAG47700.032.757736
TCGTATG2350.032.42721640
TGGTATC6850.032.0855982
ACGCAGA50900.030.6838117
CCGTTAG450.00472448930.52948624
CTTATAC3050.029.2730961
CGTATGC2850.029.19331641
CGCAGAG53700.029.083918
AGAGTAC54400.028.77106911
CTCGTAT2200.028.29818539
CAGAGTA55500.028.2008310
GCAGAGT55750.028.074379
GCCGTCT2500.027.85151146
Produced by FastQC (version 0.11.5)