FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847317
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19500.2301381891311044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7550.040.9529341
GGTATCA19750.038.094871
GTATCAA42900.037.6383061
TGGTATC8550.033.334662
TATCAAC49350.032.494792
ATCAACG51400.031.0486583
TCAACGC51650.030.8046234
GCGGTAT450.0047182430.5377461
CAACGCA52550.030.3949875
AACGCAG53000.030.3291426
CGTGATA605.592928E-428.59699627
ACGCAGA56450.028.4148277
CGCAGAG57800.027.8697578
AGAGTAC58700.027.32567411
CAGAGTA60250.026.90712210
GCAGAGT60200.026.8156059
GAGTACG41200.023.459312
AGTACGG41550.023.179213
GAGTACT28850.021.97804312
ACTGCGT800.003011539821.421178
Produced by FastQC (version 0.11.5)