FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397720
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55650.39814841312995447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25500.041.3607181
GTATCAA64600.040.5240021
GTGGTAT8300.039.7358971
TATCAAC70750.036.590642
ATCAACG75500.034.07983
TCAACGC75450.033.9810144
ATACCGA902.145608E-433.67397370
CAACGCA77950.032.964975
AACGCAG80850.031.9096836
TGGTATC10000.031.5873322
CTAGCGA450.004780851330.45692414
CGCAGAG87200.029.468118
ACGCAGA87600.029.4508867
AGAGTAC88650.029.02476711
CAGAGTA91400.028.18896910
GCAGAGT92400.027.9209739
GAGTACG49550.026.13699712
CGAGTTA809.966473E-525.71827124
AGTACGG50200.025.59381713
GAGTACT52950.024.07046712
Produced by FastQC (version 0.11.5)