FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583265
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTACCCCTCTTACTTTCGGCGGAGGGACCAAGCTGGAGATCATCCGAACTGTGGCTGCACCAT20200.34632628393611825No Hit
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAAGTCAGTTTCAGGGAGA15870.2720890161418909No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14860.254772701945085No Hit
CTATAATACTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGTAATCCGAC14300.24517157724190547No Hit
GTATATTACTGTGCGAGGCAGTGGGAGCTAAAGACATGGTTCGACCCCTGGGGCCAGGGAATCCTGGTCACCGT13400.2297411982546527No Hit
ACCTACTATTATGCGTCCCTCAAGAGCCGACTCACCATATCCGTCCACTCATCTAAGAATCAGATCTCCCTGAAA10240.175563423143854No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9730.16681954171774407No Hit
GTCTCTGGTGGCTCCATCAGCAGTACTGATTACTACTGGGCCTGGATCCG9690.16613374709608839No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9620.16493360650819097No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9500.16287622264322393No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8950.15344654659545834No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8630.14796018962221288No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8250.14144514071648392No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC8150.13973065416234473No Hit
GTAGGAGACAGAGTCACCGTCACTTGCCGGGCGAGTCAGGGCATTAGCAATTCTTTAGCCTGGTATCAGCAGAAA8100.13887341088527513No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8050.13801616760820554No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7940.13613023239865243No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7910.13561588643241065No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7880.1351015404661689No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7680.1316725673578905No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7580.1299580808037513No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7510.12875794021585385No Hit
GGTCTATGGTGCCTCCTTACTGGAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCGGGGTCGGATTACA6850.11744232895853515No Hit
GTGCAGCCACAGTTCGGATGATCTCCAGCTTGGTCCCTCCGCCGAAAGTA6690.11469915047191244No Hit
CAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACAT6450.11058438274197835No Hit
CTCTTATAGTGGGACGACCTACTATTATGCGTCCCTCAAGAGCCGACTCA6390.10955569080949483No Hit
TGTCAGGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTC6330.10852699887701131No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6240.10698396097828602No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTACTGATTACTACTGGG6230.10681251232287212No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6210.10646961501204427No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA6160.10561237173497466No Hit
ATAGTAGGTCGTCCCACTATAAGAGATACTGCCAATCCACTCCAGGCCCTTCCCTGGGGGCTGTCGGATCCAGGC6010.10304064190376587No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT5920.10149760400504058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAT4150.066.916370
GTGGTAT2350.046.896581
GGACGGA4650.039.98760669
GTATCAA17650.037.4641271
GCGGTCA4450.033.2816423
TATCAAC20300.032.5734862
ACCGTAC851.2226155E-732.4221527
CTCGTAT751.6367267E-632.27654339
TCAACGC20700.031.7776724
ATCAACG20750.031.7011013
GGCGGTC4700.031.51404622
CAACGCA21000.031.4877055
TTACACG553.2886505E-431.3087964
CGTGTCT4950.031.30074513
CCGTGTC4650.031.09880412
CGTATGC901.9336585E-730.77136241
ACGGGTG450.004660569630.6130475
GTAATAC450.004660569630.6130473
AACGCAG21650.030.5423456
AGTGGAC4950.030.22721766
Produced by FastQC (version 0.11.5)