FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583265
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTACCCCTCTTACTTTCGGCGGAGGGACCAAGCTGGAGATCATCCGAACTGTGGCTGCACCAT18950.3248952020093782No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14690.2518580748030484No Hit
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAAGTCAGTTTCAGGGAGA13580.23282727405210327No Hit
CTATAATACTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGTAATCCGAC12060.20676707842918743No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11220.19236539137441813No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10870.18636468843493095No Hit
GTATATTACTGTGCGAGGCAGTGGGAGCTAAAGACATGGTTCGACCCCTGGGGCCAGGGAATCCTGGTCACCGT10560.18104978011709943No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9240.158418557602462No Hit
GTCTCTGGTGGCTCCATCAGCAGTACTGATTACTACTGGGCCTGGATCCG8690.14898888155469642No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8680.14881743289928248No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8680.14881743289928248No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8410.14418831920310665No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8180.14024500012858648No Hit
ACCTACTATTATGCGTCCCTCAAGAGCCGACTCACCATATCCGTCCACTCATCTAAGAATCAGATCTCCCTGAAA7770.13321560525661577No Hit
GTAGGAGACAGAGTCACCGTCACTTGCCGGGCGAGTCAGGGCATTAGCAATTCTTTAGCCTGGTATCAGCAGAAA7690.13184401601330442No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7630.1308153240808209No Hit
TGTCAGGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTC7390.1267005563508868No Hit
GGTCTATGGTGCCTCCTTACTGGAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCGGGGTCGGATTACA6950.11915681551267433No Hit
CAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACAT6820.11692798299229339No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6810.11675653433687948No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6760.11589929105980987No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6700.11487059912732635No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6340.10869844753242522No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTACTGATTACTACTGGG6240.10698396097828602No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6200.10629816635663034No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC6140.10526947442414682No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6130.10509802576873291No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6110.10475512845790508No Hit
GTCAGGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCC6050.10372643652542156No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)