FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1418610
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTAGCAGATACA26170.18447635361374867No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22970.16191906161665293No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG21870.15416499249265125No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16590.11694546069744326No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACTTTTTAAATTGGTATCAGCAGAAA16100.11349137536038797No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16060.11320940921042427No Hit
GTATGATGATCTCCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT16020.1129274430604606No Hit
CCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG15930.11229301922304229No Hit
GTCCAAGGGAGATCATCATACTGTTGACAGTAATATGTTGCAATATCTTC15800.11137662923566026No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15750.11102417154820562No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15740.11095368001071472No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15670.11046023924827825No Hit
TCATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGTAGGCTGCTGATGGTGAAAGTAAAATCTGTC14930.10524386547394984No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14410.10157830552442179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6450.043.2633971
GTATCAA45900.038.653591
CGTATGC1750.037.60757441
TCGTATG1700.036.66311640
TATCAAC51650.034.350432
CGAATAA800.003663924332.15688370
ATCAACG55550.032.0019343
TCAACGC55700.031.792054
CAACGCA57800.030.7561865
AACGCAG58350.030.5253266
CGCAGAG63350.028.161518
CTCGTAT1850.028.06541639
ACGGGTA1353.1286618E-1028.06383716
GGTATCA30950.027.6049081
ACGCAGA64750.027.5003917
AGAGTAC65500.027.34132611
CAGAGTA66100.027.09314310
GCAGAGT67200.026.5993429
TATACAC2900.024.9477793
TATGCCG2650.024.85281243
Produced by FastQC (version 0.11.5)