FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1418610
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23100.16283545160403493No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTAGCAGATACA22880.1612846377792346No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22310.15726662014225193No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG21730.1531781109677783No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19020.13407490430773786No Hit
GTATGATGATCTCCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT15060.10616025546133186No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACTTTTTAAATTGGTATCAGCAGAAA14720.10376354318664045No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14720.10376354318664045No Hit
GTCCAAGGGAGATCATCATACTGTTGACAGTAATATGTTGCAATATCTTC14250.10045044092456701No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14240.10037994938707608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6500.046.1143721
GTATCAA48000.039.047041
TATCAAC54100.034.4925652
TCAACGC56400.032.7263954
ATCAACG56550.032.6626243
AACGCAG58500.031.5936436
CAACGCA58350.031.557115
CGCAGAG63450.029.1288918
ACGCAGA64500.028.601447
AGAGTAC65150.028.52611
GGTATCA31550.028.5017531
GCAGAGT65900.028.0980829
CAGAGTA67050.027.82110410
AGTACGG46850.024.41874113
GAGTACG47400.024.27864512
GAGTACT29150.024.27653512
AGTACTT29850.023.59381513
ATAAACG902.1705731E-422.9307443
TACGGGG26050.022.02949515
GTACGGG54250.021.08863314
Produced by FastQC (version 0.11.5)