FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600155
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13150.2191100632336646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3600.042.076751
GTATCAA26850.040.773211
GGTATCA10750.039.7104951
TATCAAC30150.036.0610282
TGGTATC4150.035.649932
ATCAACG31650.034.5463263
TCAACGC32400.033.9305884
CAACGCA33050.033.6762545
AACGCAG33500.033.4261866
ACGCAGA35700.031.2700947
CGCAGAG36550.030.5428838
AGAGTAC35950.030.38381411
CAGAGTA37050.029.94527810
GCAGAGT38100.029.3904789
GAGTACG24350.028.07166312
AGTACGG24150.027.73521413
TAGCGTC500.00776849227.57338334
TTGGCGC700.001259130924.8853849
TACGGGG11800.024.45590215
GTACGGG27050.024.38080414
Produced by FastQC (version 0.11.5)