FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974648
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12410.12732801996207863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19050.040.3353731
GTGGTAT5800.039.0345341
GTATCAA44400.038.8613621
TATCAAC51550.033.604372
TGGTATC6950.033.562722
ATCAACG54350.031.8100263
TCAACGC54550.031.6305164
CAACGCA55950.030.9616665
AACGCAG57650.029.9891576
ACGCAGA61500.028.1603377
CGCAGAG61950.027.9557868
TCGCTAG500.00795342427.4424571
AGAGTAC64300.026.93407211
CAGAGTA65050.026.62353510
GCAGAGT65250.026.541939
GAGTACG38750.024.16086812
AGTACGG39250.023.76571513
ACGAGAC2950.023.25274322
TACGGGG14550.022.3914515
CGAGACG3000.021.72416523
Produced by FastQC (version 0.11.5)