FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264133
Sequences flagged as poor quality0
Sequence length20-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35500.28082488155913976No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14770.11683897184868997No Hit
GTATCAACGCAGAGTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTC13760.10884930620433135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69350.043.000671
GGTATCA25250.042.4898721
TATCAAC77350.038.27832
GTGGTAT7550.038.135971
ATCAACG81200.036.4503863
TCAACGC82000.036.069064
CAACGCA83450.035.470735
AACGCAG87050.034.239686
TGGTATC8450.034.066052
CGCAGAG93700.031.8461728
ACGCAGA93850.031.7952737
AGAGTAC97700.030.7162511
CAGAGTA98300.030.59959810
GCAGAGT98550.030.3830789
AGTACGG53500.026.73745213
GAGTACG53800.026.65091112
GAGTACT59750.026.23058112
AGTACTT60150.025.82960513
GTACTTT64050.024.04597714
AAGTCCG1801.6569616E-523.96767270
Produced by FastQC (version 0.11.5)