FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2646770
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36340.13729942533729791No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32350.12222444715634527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41350.041.736481
GTATCAA93550.039.0683631
TATCAAC107350.033.9498022
CGCTAAT3400.033.47831332
GTGGTAT16900.033.2241971
ATCAACG112750.032.3543743
TCAACGC113700.032.0234534
CAACGCA116750.031.216375
AACGCAG118650.030.745526
TGGTATC17750.030.27472
TCGCTAA4000.030.17435631
TCGTATG3450.030.08884240
ACGCAGA129300.028.2003237
CGCAGAG130500.028.0196328
CGTATGC4150.027.5327541
CAGAGTA136950.026.6999810
GCAGAGT139300.026.2495489
AGAGTAC142750.025.59102611
AGTACGG85850.023.18120413
GAGTACG86700.023.07307412
Produced by FastQC (version 0.11.5)