FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2646770
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45050.17020746041401408No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31640.11954193224194018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43000.040.454661
GTATCAA99800.038.3813171
GTGGTAT17650.035.1296161
TGGTATC17650.034.5251582
TATCAAC110800.034.2101332
ATCAACG115750.032.8449823
TCAACGC115900.032.628124
CAACGCA119000.031.8202865
AACGCAG121450.031.3481316
ACGCAGA131550.028.91527
CGCAGAG133750.028.3368238
CAGAGTA138050.027.62841410
GCAGAGT139200.027.3261099
AGAGTAC144400.026.3886611
AGTACGG93400.024.6880113
GAGTACG94400.024.64117812
AGTACTT67400.021.8219913
TACGGGG47200.021.77481715
TTCGGGG13800.021.7071170
GTACGGG108300.021.13635314
Produced by FastQC (version 0.11.5)