FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789657
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTACTCCTCGTACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT48410.6130509828951051No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42860.5427673027656311No Hit
GTTTTATACAACTCCAACAATAAGAACTTCTTAGCTTGGTACCAGCAGAA37450.4742565442970809No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29640.3753528430698392No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29310.3711738134405191No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27660.35027866529391877No Hit
ACTTAGGAGGCTGTCCTGCTTTCTGCTGGTACCAAGCTAAGAAGTTCTTATTGTTGGAGTTGTATAAAACACT26580.33660184105250757No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26530.33596865474503484No Hit
ATACTACTCCTCGTACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA22860.28949277977653587No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22850.28936614251504134No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22410.28379410300928126No Hit
ATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCACCATCAGCAGCC21030.2663181609230337No Hit
CTCCTAAGTTGCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCT21000.2659382491385501No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20970.26555833735406636No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19380.2454230127764333No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19170.24276363028504783No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCAC18830.23845796339423317No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18260.23123963948904397No Hit
GTCTCCAGACTCCCTGGCCGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA17940.22718724712121846No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG17480.22136193309246927No Hit
GTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT16750.2121174130033673No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16330.2067986480205963No Hit
GTTTATTACTGTCAGCAATATTATACTACTCCTCGTACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA16040.2031261674372544No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15930.2017331575608144No Hit
AAGCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCTTCTACCCGGGAA15280.1935017355636688No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTA14280.18083800941421402No Hit
GTCAGGGACCCCGGATTCCCGGGTAGAAGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGCTTTCTGCTGGTA14250.18045809762973036No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13960.17678561704638848No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC13910.17615243073891576No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13790.1746327836009812No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13780.17450614633948663No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13760.17425287181649754No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13730.17387296003201388No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13370.1693140186182102No Hit
CTCCCTGGCCGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAACTCCAA13150.16652799886533012No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAACTCCAACAATAAGAACTT13080.1656415380348683No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12360.1565236552072609No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA12080.15297781188541354No Hit
GTCTGAGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG12050.1525979001009299No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12050.1525979001009299No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12040.15247126283943535No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11740.1486721449945989No Hit
CTTCTTAGCTTGGTACCAGCAGAAAGCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCTTCTACCCGGGAATC11500.14563285071872978No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11470.14525293893424615No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11400.1443664781037843No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACGGCCAGGGAGTCTGGAGA11390.14423984084228977No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11380.1441132035807952No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACTCCTCGTACTTTTGGCCAGGGGACCAAG11330.14348001727332246No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11320.14335338001182793No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11100.1405673602589479No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11050.13993417395147514No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10550.13360231087674773No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10440.13220930100030773No Hit
CCCTGGCCGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC10330.1308162911238677No Hit
GGATCTCTGGTGCCTGCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCCGTGTCTCTGGGCGAGAGGG10310.1305630166008786No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10230.12954991850892222No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10150.12853682041696585No Hit
GACCGATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCACCATCAG10000.12663726149454763No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACT9950.12600407518707488No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9910.12549752614109672No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT9860.12486433983362397No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9680.1225848691267221No Hit
GTACCAGCAGAAAGCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCTGA9620.12182504555775482No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9580.12131849651177663No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAACTCCAACAATAA9580.12131849651177663No Hit
GCAGAAAGCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCTGACCGATT9500.12030539841982024No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTGCGGGGACATCGTGATGA9420.11929230032786386No Hit
GTCCTGCTTTCTGCTGGTACCAAGCTAAGAAGTTCTTATTGTTGGAGTTG9410.11916566306636933No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9390.11891238854338022No Hit
GAGTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT9240.117012829620962No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9230.11688619235946747No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9160.11599973152900563No Hit
TTATACTACTCCTCGTACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT9150.11587309426751109No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9120.11549318248302745No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9000.11397353534509287No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA8840.1119473391611801No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8830.11182070189968554No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8820.11169406463819101No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGAAGCCCAGTAAAT8630.10928795666979461No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8620.10916131940830005No Hit
GTCTATGGTGCGTCCTTCAGTGGTTACTACTGGAGTTGGATCCGCCAGCC8540.10814822131634368No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8390.10624866239392547No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA8290.10498228977898No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8250.1044757407330018No Hit
CCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAACTCCAACAATAAG8160.10333600537955087No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8100.10257618181058359No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC7970.10092989741115448No Hit
TTATTACTGTCAGCAATATTATACTACTCCTCGTACTTTTGGCCAGGGGA7930.10042334836517627No Hit
CTCCAGACTCCCTGGCCGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC7910.10017007384218718No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC7900.10004343658069263No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)