FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789657
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTACTCCTCGTACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT49620.6283740915359454No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43460.550365538455304No Hit
GTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT40430.5119944482224561No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28290.35825681276807525No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25830.3271040464404166No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24130.3055757119863434No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24010.30405606484840886No Hit
GTTTTATACAACTCCAACAATAAGAACTTCTTAGCTTGGTACCAGCAGAA23970.30354951580243067No Hit
ATACTACTCCTCGTACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA22460.284427289316754No Hit
ACTTAGGAGGCTGTCCTGCTTTCTGCTGGTACCAAGCTAAGAAGTTCTTATTGTTGGAGTTGTATAAAACACT22060.2793617988569721No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21500.2722701122132774No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20320.25732691535692076No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTA20130.25492080738852435No Hit
GAGTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT19920.2522614248971389No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19350.24504310099194965No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19200.24314354206953145No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18870.23896451244021139No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG17180.21756281524763282No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCTCAGACCCGCTGCCA17040.2157898935867092No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCAC16480.2086982069430145No Hit
GTTTATTACTGTCAGCAATATTATACTACTCCTCGTACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA15710.19894713780793435No Hit
CTCCTAAGTTGCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCT15480.19603448079355976No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15360.19451483365562516No Hit
ATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCACCATCAGCAGCC15350.1943881963941306No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15270.19337509830217423No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15070.19084235307228328No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15030.1903358040263051No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15010.190082529503316No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14770.18704323522744684No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC14560.18438385273606134No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14410.18248429381364312No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCTCAGACCCGCTGCCACTGAATCGGTCAGGGACC14370.18197774476766496No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC12730.16120923388255914No Hit
GTCTCCAGACTCCCTGGCCGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA12550.15892976317565727No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12550.15892976317565727No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12390.15690356699174451No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12390.15690356699174451No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12360.1565236552072609No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12190.15437082176185357No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12170.15411754723886448No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12170.15411754723886448No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12150.15386427271587538No Hit
GTCTGAGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG11600.14689922333367525No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11420.1446197526267734No Hit
GTCAGGGACCCCGGATTCCCGGGTAGAAGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGCTTTCTGCTGGTA11380.1441132035807952No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11210.1419603701353879No Hit
GTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT10680.13524859527617686No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10660.1349953207531878No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACTCCTCGTACTTTTGGCCAGGGGACCAAG10630.1346154089687041No Hit
AAGCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCTTCTACCCGGGAA10550.13360231087674773No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACT10060.12739708506351494No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9990.1265106242330531No Hit
CTCCCTGGCCGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAACTCCAA9960.12613071244856944No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9880.12511761435661306No Hit
GTCTCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT9850.12473770257212943No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA9840.12461106531063487No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9620.12182504555775482No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCTCAGACCCGCTGCCA9550.12093858472729299No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9500.12030539841982024No Hit
TTATACTACTCCTCGTACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT9360.11853247675889658No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9260.11726610414395111No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9210.11663291783647836No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9170.11612636879050019No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA9140.11574645700601653No Hit
GGCCAAAAGTACGAGGAGTAGTATAATATTGCTGACAGTAATAAACTGCC8980.11372026082210376No Hit
GAGTGAAATCTGTCTCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG8870.11232725094566375No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT8420.10662857417840911No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8390.10624866239392547No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8360.10586875060944181No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8300.10510892704047453No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT8290.10498228977898No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8290.10498228977898No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8260.10460237799449636No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8000.10130980919563812No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7990.10118317193414356No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT7980.10105653467264901No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7910.10017007384218718No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC7900.10004343658069263No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAC200.007524494551.458278
TGGTATC3350.049.2508432
GTGGTAT3550.046.5056571
GGTATCA10300.044.078781
TACTGAT4150.042.14462770
GTATCAA26550.039.9005661
TATCAAC29950.034.8894422
TTTCGGC400.00266466734.3163815
TGTCTAC2750.032.79185566
ATCAACG32100.032.748073
TCAACGC32100.032.5113564
CGTTGAT851.2473538E-732.3510482
CAACGCA33050.031.6626555
TCGACGT2550.031.54341960
AACGCAG33850.031.1131276
TTCGACG2700.031.05819959
GTCTACT3300.029.91700767
CTACTGA4050.029.90569369
TCTACTG3550.029.26378468
CGCAGAG36850.028.9525538
Produced by FastQC (version 0.11.5)