FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1673638
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60620.3622049690554349No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA42100.25154782575443435No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATT41110.24563256809417566No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40100.23959781027916432No Hit
GAGTATTAACAGCGACTTAATCTGGTATCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC37480.22394329000656057No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT35830.21408452723946278No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35220.2104397725195054No Hit
GCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCGACTGGCCTACTTTCGGCCCTGGGACCA34390.20548051609726833No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34020.2032697632343434No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32680.19526325286591248No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32070.1916184981459551No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAACAGCGACTTAATCTGGTATCAACAGAAACCTGGCCAGGCTCCCAGGCT30890.18456798901554577No Hit
GTATTAACAGCGACTTAATCTGGTATCAACAGAAACCTGGCCAGGCTCCC29570.17668097880186756No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAACAG29450.17596397787335136No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28410.16974996982621093No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27780.16598571495150088No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27130.1621019599220381No Hit
GTCCCAGACCCACTGCCACTGAATCTGGCTGGGATGCCAGTGGCCCTCTT26890.1606679580650057No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT26160.15630620241653212No Hit
GTGCAGCCACAGTTCGCTTGAAATCCACTGTGGTCCCAGGGCCGAAAGTA25800.1541551996309835No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24620.1471046905005742No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24320.1453121881792837No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA23880.14268318477472428No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAGGTCTTCTGGAGGCGCCTTCAGTAA23700.14160768338194998No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGAAATCCACTGTGGTCC23220.13873967966788517No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22980.13730567781085276No Hit
TCTATGATGCATCCAAGAGGGCCACTGGCATCCCAGCCAGATTCAGTGGCAGTGGGTCTGGGACAGACTTCACT22940.13706667750134738No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22460.13419867378728256No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22310.1333024226266373No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCGACTGG22230.1328244220076265No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21660.12941866759717452No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21570.12888091690078737No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21500.12846266635915293No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21220.12678966419261511No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20990.12541541241295906No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20020.11961965490745312No Hit
GTTTATTACTGTCAGCAGCGTAGCGACTGGCCTACTTTCGGCCCTGGGAC20020.11961965490745312No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19530.11669190111601195No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT19230.11489939879472143No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18910.11298739631867823No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18840.11256914577704377No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18540.11077664345575328No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18430.11011939260461343No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18250.10904389121183912No Hit
CATCTATGATGCATCCAAGAGGGCCACTGGCATCCCAGCCAGATTCAGTGGCAGTGGGTCTGGGACAGACTTCA18190.10868539074758102No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17850.10665388811678511No Hit
GTATCAACGCAGAGTACGGGGAGGAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTT17790.10629538765252701No Hit
GTTAATACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGGCTGGAGA17740.10599663726564526No Hit
GTAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17680.10563813680138717No Hit
ATCCAAGAGGGCCACTGGCATCCCAGCCAGATTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAG17620.10527963633712904No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC17300.10336763386108584No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT17270.1031883836289568No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17250.10306888347420409No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17190.102710383009946No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAATCTGGCTGGGATGCCA17190.102710383009946No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17130.1023518825456879No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17080.10205313215880614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)