FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871946
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5350.040.4167441
GTATCAA34300.038.825061
GGTATCA15150.034.2088781
TATCAAC39400.033.973712
ATCAACG41450.032.5418783
TCAACGC41400.032.4982724
CAACGCA42350.031.8503135
CCGCTAT1202.6227435E-531.27715770
TGGTATC7000.030.8899382
AACGCAG43850.030.7607926
CGAATAA450.004644091730.63688934
ACGCAGA47300.028.433167
CGCAGAG47250.028.3180268
AGAGTAC48000.028.01850711
TCGTATG755.993992E-527.71209540
CAGAGTA49250.027.30737910
ACGAGAC2150.027.14000722
GCAGAGT50500.026.5635179
TAAGGCG2050.025.14117829
GACTAAG2050.025.12097726
Produced by FastQC (version 0.11.5)