FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871946
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17300.1984067820713668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16650.040.019071
GTGGTAT6400.039.176211
GTATCAA38450.038.231991
TGGTATC6700.036.890342
TATCAAC43250.033.6538432
TCAACGC44500.032.3534774
ATCAACG45200.032.2556763
CAACGCA45550.031.5288095
AACGCAG47000.030.7713586
ACGCAGA49750.029.070437
CGCAGAG51200.028.180218
CAGAGTA51800.027.72147610
AGAGTAC51600.027.69608911
GACCGGA1156.863786E-427.6099270
GCAGAGT53200.026.9919629
TGGACCG1951.8189894E-1224.6079435
AGTACGG34600.024.56448613
GAGTACG34700.024.49369212
ACGGTTA800.002689336421.8430250
GTACGGG38500.021.54327214
Produced by FastQC (version 0.11.5)