FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481388
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34890.23552236146100822No Hit
CTATAACAGTTATCCGTGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT29540.1994075826184632No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT26550.17922380902234933No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25100.16943569139212686No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC24310.16410285489014356No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23970.16180771006650518No Hit
CACTATCACCAGCCTGCAGCCTGATGATGTTGCAACTTATTACTGCCAACACTATAACAGTTATCCGTGGACCTT23590.15924254820479172No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23570.15910753968575417No Hit
CTGTTATAGTGTTGGCAGTAATAAGTTGCAACATCATCAGGCTGCAGGCTGGTGATAGTGAGAGTGAATTCTGTC23420.15809497579297255No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22430.15141205410061376No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22250.1501969774292758No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21920.1479693368651562No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21070.14223147480606027No Hit
CTCTAAGACTGTTCATTTGCAGATACAAGGTGTTCTTGGAATTGTCTCTG20880.14094889387520354No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20880.14094889387520354No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19980.13487351051851373No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19660.13271337421391288No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG19020.12839310160471126No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18560.12528790566684758No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18270.12333028214080309No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT18270.12333028214080309No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18050.12184518843139003No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTGTTAGTAAGTGGTTGGCCTGGTATCAGCAAAAA17890.12076512027908962No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17820.12029259046245817No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA17650.119145018050639No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17330.11698488174603817No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16120.10881686634426632No Hit
CTCCTGTGAAGCCTCCGGATTCAGTTTTAAGGACTATGCCATGAATTGGGTCCGCCAGGCTCCAGGGGAGGGA15680.1058466789254402No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15680.1058466789254402No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15440.10422657669698956No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGC15390.1038890553993957No Hit
CACTATAACAGTTATCCGTGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT15160.10233645743046386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5800.052.8512841
TATCGCG350.001338652339.4840836
GTATCAA51450.039.2288671
TATCAAC56700.035.292842
ATCAACG60450.033.160433
TCAACGC61000.032.8049854
CAACGCA62200.032.338215
AACGCAG62850.032.0037656
CGCAGAG68550.029.3799698
AGAGTAC69600.029.08512911
ACGCAGA70350.028.5793067
CAGAGTA70950.028.4831910
GCAGAGT71150.028.3547389
AGTACGG46150.026.10954313
GAGTACG46450.026.08914412
AGTCGCG700.001313760124.7076839
TAACGGA10400.023.39070370
GAGTACT35000.023.1154712
TGTTAGA6650.023.10375870
GTGTCCA17800.022.65758170
Produced by FastQC (version 0.11.5)