FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481388
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31910.21540609212441306No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28720.19387223333792364No Hit
CTGTTATAGTGTTGGCAGTAATAAGTTGCAACATCATCAGGCTGCAGGCTGGTGATAGTGAGAGTGAATTCTGTC26790.18084391125079993No Hit
CTATAACAGTTATCCGTGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT26510.1789537919842742No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT24210.16342781229495582No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24120.16282027395928683No Hit
CACTATCACCAGCCTGCAGCCTGATGATGTTGCAACTTATTACTGCCAACACTATAACAGTTATCCGTGGACCTT22320.15066950724590722No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21560.14553918352248027No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20710.13980132146338434No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20600.1390587746086778No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20490.1383162277539713No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20320.13716865534215208No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19490.1315658018020937No Hit
CTCTAAGACTGTTCATTTGCAGATACAAGGTGTTCTTGGAATTGTCTCTG18730.12643547807866676No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18450.12454535881214104No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18030.12171017991235247No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18010.1215751713933149No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17780.12002257342438308No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17100.11543228377710633No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16530.11158454098453613No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT16370.11050447283223572No Hit
CTCCTGTGAAGCCTCCGGATTCAGTTTTAAGGACTATGCCATGAATTGGGTCCGCCAGGCTCCAGGGGAGGGA15080.10179642335431366No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14960.10098637224008834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5750.044.9326441
GTATCAA48200.041.3809131
TATCAAC53700.036.217592
TCAACGC57200.034.08154
ATCAACG57100.034.0414543
CAACGCA59050.033.110075
AACGCAG60250.032.5076456
CGCAGAG64600.030.3186658
ATGCCGT2002.3143002E-829.59063170
AGAGTAC65850.029.53441611
CAGAGTA66650.029.2830210
ACGCAGA67200.029.196757
GCAGAGT66800.029.062959
TAACGGA10700.027.65479770
GATAGAC500.00779237127.5586871
CGCAATT500.00785562527.51306721
CTAATAG1554.1836756E-1127.2008163
GCGCAAA1000.00977979326.30278470
ACGGGTA1608.185452E-1125.7839216
AGTACTT31600.024.79389813
Produced by FastQC (version 0.11.5)