FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1807154
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25910.14337461002216745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10500.044.8239061
GGTATCA32000.041.761691
GTATCAA76050.039.1650161
TGGTATC12700.036.5181272
TATCAAC88850.033.522792
ATCAACG93300.031.997543
TCAACGC93350.031.9435984
CAACGCA95400.031.3291995
AACGCAG100000.029.8880566
ACGCAGA106950.027.9396387
CGCAGAG108550.027.5910978
CTTATAC7500.026.5671621
AGAGTAC112900.026.5280211
ATCTCCG7050.026.30794510
CAGAGTA114350.026.25170910
GCAGAGT115100.026.0508089
ACGAGAC6900.025.89218922
GCGGTAT700.00136749724.5386351
CGAGACT7400.023.67908923
CCCACGA7850.023.1938419
Produced by FastQC (version 0.11.5)