FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1807154
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62820.34761840994182014No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18350.10154087587444126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10550.043.3163951
GGTATCA32850.042.4662361
GTATCAA81050.040.740231
TGGTATC12350.036.42342
TATCAAC94000.035.178282
ATCAACG98650.033.4959643
TCAACGC99600.033.06034
CAACGCA101600.032.558475
AACGCAG106050.031.5478346
ACGCAGA113450.029.3389917
CGCAGAG113800.029.2186348
AGAGTAC117400.028.20508611
CAGAGTA118000.028.12054610
GCAGAGT118400.028.0834489
GAGTACG67200.024.78913712
AGTACGG67850.024.65473713
GCGAATT1300.001585481123.92198870
AGTACTT69450.023.54379813
ACGGGTA3500.023.5122316
GAGTACT70700.023.2225812
Produced by FastQC (version 0.11.5)