FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264459
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37840.2992584180269981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9350.041.1231541
GTATCAA61900.039.5438461
GGTATCA27150.036.9226651
TGGTATC10850.036.0507352
TATCAAC69900.034.9004822
ATCAACG72600.033.440873
TCAACGC72400.033.404124
CAACGCA73400.033.0266845
AACGCAG75800.031.9797236
ACGCAGA81650.029.7303947
CGCAGAG82100.029.567448
GCGAACT953.403875E-728.85268823
AGAGTAC85400.028.54517711
GCAGAGT86950.028.0756999
CAGAGTA87450.027.99347710
AGTACGG44500.026.54735413
GAGTACG44850.026.18541512
TACGGGT7650.025.51887115
CGAACTA1101.2210621E-624.9261224
ACGGGTA1651.2551027E-1024.90935716
Produced by FastQC (version 0.11.5)