FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539083
Sequences flagged as poor quality0
Sequence length20-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8830.16379666952955296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC4100.042.7216262
GTATCAA27650.039.3754581
GGTATCA11250.039.0767941
GTGGTAT4600.038.8245961
TATCAAC31800.034.236832
ATCAACG33550.032.2462733
TCAACGC33700.032.102744
CAACGCA34250.031.5872215
AACGCAG35400.030.5610836
CGCAGAG38100.028.2999428
ACGCAGA38300.028.1547767
AGAGTAC40100.026.97410611
GCAGAGT40200.026.736179
CAGAGTA40600.026.55733710
AGTACGG24050.024.98640813
GAGTACG24100.024.5071212
CGGGCTA902.1901666E-422.89655717
ACGGGAG5050.022.44316116
CTTATAC2005.638867E-1122.3241441
GTACGGG27150.022.00697114
Produced by FastQC (version 0.11.5)