FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539083
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11420.2118412192556619No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5750.10666261039580176No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA405.205384E-542.829577
GTATCAA29950.041.5294041
GGTATCA11850.040.3032761
GTGGTAT4400.039.8255161
TGGTATC4350.039.453052
TATCAAC34800.035.705012
ATCAACG36700.033.4575463
TCAACGC36650.033.300564
CAACGCA37200.032.789955
AACGCAG38250.032.1585736
ACGCAGA41200.029.8559557
CGCAGAG41100.029.845238
AGAGTAC42250.029.03287311
CAGAGTA42550.028.90870310
GCAGAGT43250.028.2031529
GAGTACG24550.027.21553212
AGTACGG24600.027.02093313
ACGGGCT2450.026.57674616
TACGGGA9200.026.07259615
TACGGGC4350.025.99553115
Produced by FastQC (version 0.11.5)