FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142920
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42130.3686172260525671No Hit
GTATTATAACTGGCTCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT34430.30124593147376894No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA29100.25461099639519824No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27460.2402617856017919No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25760.2253876036817975No Hit
GTTATAATACTGCTGACAGTAATAAAGTGCAAAGTCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT25600.22398768067756272No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGACT25180.22031288279144648No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24860.21751303678297693No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24540.2147131907745074No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24140.2112133832639205No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGACTTTGCACTTTATTACTGTCAGCAGTATTATAACTGG21090.18452735099569526No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20410.17857767822769746No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20260.17726525041122737No Hit
GTCTGAAGACTTTGCACTTTATTACTGTCAGCAGTATTATAACTGGCTCT19660.172015539145347No Hit
TTATTACTGTCAGCAGTATTATAACTGGCTCTGGACGTTCGGCCAAGGGA19340.16921569313687745No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCGGCTTGGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT19320.16904070276134814No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG18990.16615336156511393No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC18220.1594162321072341No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17230.15075420851853147No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16920.14804185769782663No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCGGCTTGGCCTGGTA16380.14331711755853427No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15960.139642319672418No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC15740.13771742554159522No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15650.13692996885171316No Hit
GTGTTAGCAGCGGCTTGGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTACGATGCATCCA14900.13036782976936268No Hit
GTCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACC14430.12625555594442306No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13460.11776852273124977No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT13030.11400622965736884No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATTCCAGTGGCCCTGGT12980.11356875371854548No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12880.11269380184089875No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12690.11103139327336997No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12520.10954397508137052No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12380.10831904245266509No Hit
GAGTGTTAGCAGCGGCTTGGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTACGATGCATC12150.10630665313407761No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11920.10429426381549016No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11700.10236936968466735No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11670.10210688412137332No Hit
CAGTAATAAAGTGCAAAGTCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA11640.10184439855807931No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11600.10149441780702062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15650.044.842741
GTATCAA38950.040.6281171
TATCAAC43900.036.12542
ATCAACG46300.034.252813
TCAACGC46550.034.0688554
CAACGCA47250.033.564135
AACGCAG49100.032.4396256
CGCTAGC450.004691857430.57368519
CGCAGAG52500.030.3321328
ACGCAGA53800.029.663137
AGAGTAC53450.029.5999811
CTTATAC3200.029.0261921
GCAGAGT55000.028.9534029
CAGAGTA57150.027.80398410
CTACGCT3000.027.53679528
GTGGTAT7700.027.2530691
ACTACGC3050.027.0806327
GAGTACG36000.026.65526212
AGTACGG35900.026.63370513
TATACAC3650.026.3901233
Produced by FastQC (version 0.11.5)