FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659160
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56760.3421008221027508No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17040.10270257238602666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA81250.042.956641
GTGGTAT11150.039.6662141
GGTATCA31450.038.8092541
TATCAAC93250.037.2156452
ATCAACG97250.035.6666223
TCAACGC97100.035.5570684
CAACGCA99750.034.6695565
AACGCAG103350.033.6584666
TGGTATC14000.031.5665152
ACGCAGA111600.031.2928687
CGCAGAG112400.031.0701438
AGAGTAC116250.030.04115511
CAGAGTA117750.029.5422710
GCAGAGT120500.028.868079
GAGTACT69750.026.8242112
AGTACTT70850.026.21621513
AGTACGG63850.025.28663813
GAGTACG63950.025.24557712
GTACTTT75200.024.74744614
CGATTGT1350.002023897822.93866270
Produced by FastQC (version 0.11.5)