FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138747
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46160.4053578187253183No Hit
GTATAATGTCTGGCCTCCGCCGTACACTTTTGGCCAGGGGACCAAGCTGG33720.29611494036866837No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT26170.22981399731459226No Hit
ACATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC25990.2282333125795282No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25670.22542320638385874No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC25600.22480849565355604No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25290.2220862052765013No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24790.21769541434576775No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24530.21541220306178632No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23790.20891383248430073No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATGTCTGG23380.20531338392109924No Hit
CTGGTATCCCAGCCCGGTTCATTGGCAGTGGGTCTGGGACAGAGTTCACT21600.18968216820768793No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21200.1861695354631011No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGCGGAGGCCAGACATTATA19160.16825510846570838No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19050.16728913446094698No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA18740.16456684408389222No Hit
CATCTATGGTGCATCCATCAGGGCCACTGGTATCCCAGCCCGGTTCATTGGCAGTGGGTCTGGGACAGAGTTCA18650.16377650171636018No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCGACAATGTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT18610.1634252384419015No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17900.1571903153202599No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17650.15499491985489314No Hit
GTCCCAGACCCACTGCCAATGAACCGGGCTGGGATACCAGTGGCCCTGAT15890.13953933577871117No Hit
GGCCAAAAGTGTACGGCGGAGGCCAGACATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGG15870.13936370414148183No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15830.13901244086702313No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15740.1382220984994911No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGACAATGTAGCCTGGTA15620.13716830867611507No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT15270.1340947550246016No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGA15260.13400693920598694No Hit
GTTCATTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCC14900.1308455697358588No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14820.13014304318694142No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14750.12952833245663875No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14710.12917706918218005No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14400.1264547788051253No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC14270.1253131731631346No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC14140.12417156752114386No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14050.12338122515361183No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGCGGAGG14020.12311777769776781No Hit
ATGTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATC13740.12065893477655704No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13640.11978077659041034No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13150.11547780147829148No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13110.11512653820383281No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13030.11442401165491543No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12980.1139849325618421No Hit
GTTTATTACTGTCAGCAGTATAATGTCTGGCCTCCGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA12830.11266769528262204No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12790.11231643200816335No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12740.11187735291509002No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12450.10933069417526457No Hit
CTGCTACTCTGGCTCCCAGTTTCAGATACCACTGGAGAAATAGTGATGAC12260.10766219362158584No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11980.10520335070037506No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11850.10406174505838434No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11830.10388611342115502No Hit
GTCAGAGTGTTAGCGACAATGTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG11780.10344703432808165No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11640.10221761286747627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)