FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699455
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24550.3509875545960784No Hit
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCGTCAGGGAGA21730.3106704505650828No Hit
GGGTAGGAGGGTGCGGGAGCATAAACCATTACAACCGCATGTCTCGCACA21230.3035220278645517No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA17980.25705728031109937No Hit
GTCTATTACTGTGCGAGACATGCGGTTGTAATGGTTTATGCTCCCGCACC17210.2460487093522814No Hit
GTCTCTGGTGGCTCCATCAGAAGTAGTAATTACTACTGGGGCTGGATCCG16420.23475420148544227No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15960.2281776526009536No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15170.2168831447341144No Hit
GTATAATAAATGGCCCATGTACACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCAT14610.20887691130951955No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14390.20573160532128587No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14210.20315817314909465No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14010.2002988040688822No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13490.19286444446032983No Hit
CTCCTACCCTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTT12970.18543008485177745No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCACCCTGCAGTCTGAAGATT12750.18228477886354377No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12240.17499338770900202No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12170.17399260853092766No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11910.17027542872665147No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT11800.16870277573253462No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGGTGCTGATGGTGAGAGTGAACTCTGTC11720.16755902810044965No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11600.16584340665232217No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11170.1596957631298654No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11000.15726529941168482No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10960.1566934255956423No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10730.15340515115339798No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10590.1514035927972493No Hit
CATCTATGGTGCATCCAACAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA10530.15054578207318556No Hit
GAGTTCACTCTCACCATCAGCACCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAAATGG10360.14811531835500497No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC10010.14311142246463318No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCT9980.1426825171026013No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC9750.139394242660357No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCTGGCCAAAAGTGTACATGGGCCATTTATTATACTG9650.13796455812025077No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9590.13710674739618703No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA9580.1369637789421764No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9220.131816914597794No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTA9110.13024426160367716No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9040.1292434824256028No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8780.1255263026213266No Hit
GTGTTAGCACCAACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCC8650.12366771271918851No Hit
CTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCA8000.11437476320849804No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAACAGGGCCACTGGTATCCCAGCCAGGTT7960.11380288939245556No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCC7830.11194429949031746No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7750.11080055185823248No Hit
TCTATGGTGCATCCAACAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT7700.11008570958817936No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7690.10994274113416876No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT7630.10908493041010502No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGTT7510.10736930896197755No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA7490.1070833720539563No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA7450.10651149823791381No Hit
GGCCAAAAGTGTACATGGGCCATTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGGTG7320.10465290833577572No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7290.10422400297374385No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCAAGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT7270.10393806606572259No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCTGGTACCAGGC7240.10350916070369072No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7120.10179353925556325No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT7080.10122166543952077No Hit
CACGTGCACTGTCTCTGGTGGCTCCATCAGAAGTAGTAATTACTACTGGG7030.10050682316946764No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)