FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699455
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21610.3089548291169553No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18960.2710681888041404No Hit
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCGTCAGGGAGA17200.2459057408982708No Hit
GTCTCTGGTGGCTCCATCAGAAGTAGTAATTACTACTGGGGCTGGATCCG17150.24519089862821766No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16530.2363268544795591No Hit
GGGTAGGAGGGTGCGGGAGCATAAACCATTACAACCGCATGTCTCGCACA16070.22975030559507043No Hit
GTCTATTACTGTGCGAGACATGCGGTTGTAATGGTTTATGCTCCCGCACC15900.22731984187688986No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15560.2224589144405287No Hit
GTATAATAAATGGCCCATGTACACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCAT15030.21488158637796567No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14570.20830503749347706No Hit
CTCCTACCCTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTT13900.19872615107476535No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13130.1877175801159474No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13120.18757461166193679No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13070.18685976939188367No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12740.18214181040953314No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCACCCTGCAGTCTGAAGATT12650.18085509432343752No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12570.17971134669135255No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGGTGCTGATGGTGAGAGTGAACTCTGTC12380.17699494606515073No Hit
GAGTTCACTCTCACCATCAGCACCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAAATGG11500.16441372211221594No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11110.15883795240580167No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10380.14840125526302622No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10200.145827823090835No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT10010.14311142246463318No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCT9810.14025205338442073No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9440.1349622205860277No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9270.13253175686784713No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCTGGCCAAAAGTGTACATGGGCCATTTATTATACTG9120.1303872300576878No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC8930.12767082943148594No Hit
CATCTATGGTGCATCCAACAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA8900.1272419240694541No Hit
CTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCA8760.12524036571330535No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8690.12423958653523101No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8260.11809194301277423No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8160.116662258472668No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCAAGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT8110.1159474162026149No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8100.11580444774860427No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8060.11523257393256177No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8010.11451773166250867No Hit
GTGTTAGCACCAACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCC7880.11265914176037056No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA7870.11251617330635996No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC7750.11080055185823248No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7720.11037164649620063No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7680.10979977268015813No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7680.10979977268015813No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA7650.10937086731812626No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7610.10879899350208376No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGTT7480.10694040359994568No Hit
CACGTGCACTGTCTCTGGTGGCTCCATCAGAAGTAGTAATTACTACTGGG7330.10479587678978634No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7300.10436697142775446No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7260.10379509761171198No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTA7250.10365212915770135No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC7220.10322322379566948No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAACAGGGCCACTGGTATCCCAGCCAGGTT7150.10222244461759512No Hit
GTATCAACGCAGAGTACGGGGCTTTCTGAGAGTCAAGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTCTT7140.10207947616358451No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG7060.10093572853149951No Hit
ATCAATTACAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAAT7060.10093572853149951No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA7020.10036385471545704No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAG300.00261967867.1654370
ACACGTT200.007486960451.5227856
TACGGAT4850.049.85475570
TTCGATA306.42764E-445.82751524
GTATCAA24300.043.510131
ATAAACG405.0905357E-542.994053
GGTATCA8800.042.26681
CCTATAC350.001367749639.3088463
TATCAAC27200.038.5902862
GCTTGTA1051.6370905E-1136.0795971
GTGGTAT4350.035.6271861
ATCAACG29850.035.374673
TCAACGC30050.035.124144
CAACGCA30300.035.03375
CTCACGG400.002462617634.87201355
AACGCAG30750.034.516086
CGGGTAG501.9054045E-434.3608117
CGCAGAG32900.032.364878
AGAGTAC33450.031.6273411
GCAGAGT34200.030.933769
Produced by FastQC (version 0.11.5)