FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1553933
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGAGCGGCGCCAACATCGGGGCAGGTTATGATGTACATTGGTACCAGCAACTTCCAGGAGCAGC34320.2208589430818446No Hit
GCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACTACTCAGGGTGTTGTCGTAGGA29210.18797464240736247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23230.1494916447491623No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22970.14781847093793618No Hit
GACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACTACTCAGG22150.14254153814868467No Hit
ACTCAGGGTGTTGTCGTAGGACTGGCAGTAATAATCAGCCTCATCCTCAG22110.14228412679311142No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21390.137650722392793No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21390.137650722392793No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTC19150.12323568648069125No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19090.1228495694473314No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17510.1126818209021882No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17140.11030076586313567No Hit
GGTTATGATGTACATTGGTACCAGCAACTTCCAGGAGCAGCCCCCAAGCTCCTCATCTATGGGAACGCCAATCGC16690.10740488811293665No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16280.1047664217183109No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCGGCGCCAACATCGGGGCAGGTTATGATGTACATTGGTACCAG16190.10418724616827109No Hit
GTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15540.10000431164020585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9800.041.793881
TGGTATC10000.041.302192
GTATCAA64800.040.420481
GGTATCA25050.039.983161
TATCAAC72150.036.1119842
ATCAACG76300.034.2831653
TCAACGC76550.034.2161644
AACGCAG79950.032.8471686
CAACGCA80600.032.5395665
ACGCAGA86650.030.3333877
CGCAGAG87050.030.1149648
AGAGTAC88050.029.7729411
CAGAGTA89000.029.41648510
GCAGAGT92750.028.4496949
ACGAGAC3100.027.75148822
CGAGACT3200.026.88598423
AGTACGG57250.025.8998913
GAGTACG58000.025.56497812
AGTACTT44900.025.05518213
GAGTACT45550.024.7731712
Produced by FastQC (version 0.11.5)