FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1826395
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTACACAGGTCCAGCAATAAGAACTACTTAGGTTGGTTCCAGCAAAA163200.8935635500535208No Hit
CTCCTAGGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT106400.5825683929270502No Hit
GCCTAGGAGGCTGTCCTGGTTTTTGCTGGAACCAACCTAAGTAGTTCTTATTGCTGGACCTGTGTAGAATACT98230.537835462755866No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT97940.5362476353691288No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC70370.3852945282920726No Hit
ATATTATAGTATTCCTCCCACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCAT69820.38228313152412263No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC68740.3763698433252391No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT60680.33223919250764483No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG57210.3132400165353059No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG57130.31280199518724044No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC52060.28504239225359246No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT49390.27042342976190803No Hit
GTGTAGAATACTCTGGCTGGACTTGCAGTTGAAGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA49240.26960213973428526No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC48440.2652219262536308No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAACTGCAAGTCCAGCCAGAGTATTCTACA47920.26237478749120535No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT44120.24156877345809644No Hit
GTGTTGCAGACCCAGGTCTTCATTTCCCTGTTGCTCTGGATCTCTGGCGC42710.23384864719844284No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG42390.23209656180618105No Hit
GTTTATTACTGTCAGCAATATTATAGTATTCCTCCCACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAA40770.22322662950785563No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG39390.2156707612537266No Hit
ACTTAGGTTGGTTCCAGCAAAAACCAGGACAGCCTCCTAGGCTGCTCATT37420.20488448555761488No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAAGTG35810.19606930592779764No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG35750.19574078991674856No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35030.1917985977841595No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGAGCGATTCAGTGGCAGCGGGTCTGGGACAGATTT34920.1911963184305695No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34200.18725412629798044No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33860.18539253556870228No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAACTGCAAGTCCAGCCAGAGTATTCTACACAGGTCCAG33860.18539253556870228No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33730.18468075087809593No Hit
GTCTCTGGGCGAGAGGGCCACCTTCAACTGCAAGTCCAGCCAGAGTATTCTACACAGGTCCAGCAATAAGAACTA33250.18205262278970322No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG32840.1798077633808678No Hit
GTAAATGAGCAGCCTAGGAGGCTGTCCTGGTTTTTGCTGGAACCAACCTA32440.17761765664054052No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTATT32420.17750815130352415No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG32380.17728914062949144No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC32190.176248839927836No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAACTGCAAGTCCAGC31970.175044281220656No Hit
GTATTCTACACAGGTCCAGCAATAAGAACTACTTAGGTTGGTTCCAGCAAAAACCAGGACAGCCTCCTAGGCTG31710.17362071183944328No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT31620.17312793782286964No Hit
GGTCCAGCAATAAGAACTACTTAGGTTGGTTCCAGCAAAAACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGG31570.17285417448032872No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31440.17214238978972238No Hit
ATAGTATTCCTCCCACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGA30870.16902148768475603No Hit
GTCCCTGAGCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC30680.16798118698310058No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA30500.16699563894995334No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA30170.16518880088918333No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT29810.1632177048228888No Hit
TACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCT29410.16102759808256156No Hit
CTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCA29360.16075383474002064No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA29050.159056502016267No Hit
GTCTACGGCGTCTCATTCAGTGATTACTACTGGAGCTGGATCCGCCAGTC28940.15845422266267703No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC28910.15828996465715248No Hit
ACCTTCAACTGCAAGTCCAGCCAGAGTATTCTACACAGGTCCAGCAATAA28530.15620936325384158No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCCCTGTT27740.15188390244169525No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27710.1517196444361707No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC26860.1470656676129753No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA26700.14618962491684437No Hit
AACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGGCATCTACCCGGGAA26650.14591586157430347No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA26160.1432329808174026No Hit
GAGTATTCTACACAGGTCCAGCAATAAGAACTACTTAGGTTGGTTCCAGCAAAAACCAGGACAGCCTCCTAGG26090.1428497121378453No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC25930.1419736694417144No Hit
ACCCGGGAATCCGGGGTCCCTGAGCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC25700.14071435806602625No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25580.14005732604392807No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25520.139728810032879No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC25260.13830524065166627No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCCCTGTTGCTCTGGATCTCTGG25160.13775771396658446No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTATTCCTC25150.13770296129807627No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG24930.13649840259089627No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA24790.13573186523178174No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG24590.1346368118616181No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT24310.13310373714338902No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC24110.13200868377322542No Hit
CCACTGAATCGCTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAAT23690.12970907169588178No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG22380.12253647212131001No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT22160.12133191341413004No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT21670.11864903265722912No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC21350.11689694726496733No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG21270.11645892591690186No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA20790.11383079782850916No Hit
GCCTCCTAGGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGAGCGATTCAGTGGCAGCGGGT20610.1128452497953619No Hit
TTATTACTGTCAGCAATATTATAGTATTCCTCCCACTTTTGGCCAGGGGA20480.11213346510475554No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCC20150.11032662704398555No Hit
CTACTTAGGTTGGTTCCAGCAAAAACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGGCATCTACCCGGGAATC20100.11005286370144465No Hit
GAACTACTTAGGTTGGTTCCAGCAAAAACCAGGACAGCCTCCTAGGCTGC20030.10966959502188738No Hit
GAGCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG20020.10961484235337919No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT19950.10923157367382193No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT19880.10884830499426465No Hit
CTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCCTAGGAGGCTGTCCTGGTTTTTGCTGGAA19530.1069319615964783No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA19450.10649394024841285No Hit
GGCCAAAAGTGGGAGGAATACTATAATATTGCTGACAGTAATAAACTGCC19380.10611067156885559No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA19300.10567265022079014No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19250.10539888687824922No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCCCTGTTGCTCTGGATCTCTGGCGCCTACGGGGACATCGTGATGA19180.10501561819869197No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC19090.10452284418211832No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19050.10430383350808559No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC19040.10424908083957743No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18690.10233273744179106No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA18660.10216847943626652No Hit
TCATTTCCCTGTTGCTCTGGATCTCTGGCGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGG18650.10211372676775836No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18500.10129243674013562No Hit
GAATCGCTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCCTAGGAGGCTGTCCTGGTTTTTG18460.1010734260661029No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG18300.10019738336997198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)