FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851826
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26840.31508782310002276No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20700.2430073747455466No Hit
TCACTAGACTGGAGCCTGAAGATTTTGCAGTCTATTACTGTCAGCAGTTT19890.23349839051637306No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18650.21894142700504562No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18490.21706310913261628No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18090.21236731445154292No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA17060.20027564314777901No Hit
CTATTACTGTCAGCAGTTTGGTAGCTCACCGCTCACTTTCGGCGGGGGGA16420.19276237165806162No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16400.19252758192400796No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16340.19182321272184694No Hit
CTCCAGGGACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC14790.17362700833268768No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14440.16951818798674848No Hit
GTCTATTACTGTCAGCAGTTTGGTAGCTCACCGCTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAA14190.16658331631107762No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13710.1609483626937896No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13100.1537872758051527No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCCGCCGAAAGTG12390.14545224024624748No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11640.13664762521923493No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11490.1348867022138324No Hit
CAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTAGTGATGGTGAGAGTGAAGTCTGTCCCCGACCCACTGCCA11460.1345345176127519No Hit
GTCTCCAGGGACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTCGCAG11240.13195183053816154No Hit
GACTTCACTCTCACCATCACTAGACTGGAGCCTGAAGATTTTGCAGTCTATTACTGTCAGCAGTTTGGTAGCTCA10550.12385158471331No Hit
GTAATAGACTGCAAAATCTTCAGGCTCCAGTCTAGTGATGGTGAGAGTGAAGTCTGTCCCCGACCCACTGCCA10550.12385158471331No Hit
CTCCCAGACTCCTCATCTATGGCGCATCCAGCAGGGCCACTGGCATCCCT10540.12373418984628316No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10320.12115150277169282No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10170.1193905797662903No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9890.11610352348953895No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC9540.11199470314359974No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9350.10976420067008989No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8670.10178134971226518No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC8650.10154655997821152No Hit
GTTCAGTGGCAGTGGGTCGGGGACAGACTTCACTCTCACCATCACTAGAC8580.10072479590902367No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8560.10049000617496999No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8550.10037261130794319No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)