FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851826
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27180.31907924857893516No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24580.28855658315195826No Hit
TCACTAGACTGGAGCCTGAAGATTTTGCAGTCTATTACTGTCAGCAGTTT19620.23032872910664853No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17030.19992345854669852No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15880.18642304883861258No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14840.17421398266782182No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14340.16834423931648013No Hit
CTATTACTGTCAGCAGTTTGGTAGCTCACCGCTCACTTTCGGCGGGGGGA14110.16564415737486293No Hit
GTCTATTACTGTCAGCAGTTTGGTAGCTCACCGCTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAA14060.16505718303972877No Hit
CTCCAGGGACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC13890.1630614703002726No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13550.15907004482136022No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12390.14545224024624748No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11090.13019090753275903No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10910.12807779992627602No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCCGCCGAAAGTG10860.12749082559114186No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10790.12666906152195404No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10630.12479074364952468No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10580.1242037693143905No Hit
GACTTCACTCTCACCATCACTAGACTGGAGCCTGAAGATTTTGCAGTCTATTACTGTCAGCAGTTTGGTAGCTCA10220.11997755410142447No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10110.11868621056412929No Hit
CTCCCAGACTCCTCATCTATGGCGCATCCAGCAGGGCCACTGGCATCCCT9910.1163383132235926No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9620.11293386207981441No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9360.10988159553711674No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9250.10859025199982156No Hit
GTAATAGACTGCAAAATCTTCAGGCTCCAGTCTAGTGATGGTGAGAGTGAAGTCTGTCCCCGACCCACTGCCA9210.10812067253171422No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9200.10800327766468738No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9110.10694672386144588No Hit
GTCTCCAGGGACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTCGCAG9090.10671193412739222No Hit
CAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTAGTGATGGTGAGAGTGAAGTCTGTCCCCGACCCACTGCCA9070.10647714439333854No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9030.1060075649252312No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8950.10506840598901654No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8880.10424664191982869No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8720.10236832404739934No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)