FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134855
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19480.17165188504258252No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12670.11164421886496513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22300.043.1448331
GTATCAA56400.040.6979941
GTGGTAT7800.038.767131
TATCAAC63050.036.3205032
TGGTATC8350.034.949712
TCAACGC66900.034.1247524
ATCAACG67050.033.9836163
CAACGCA69000.033.021945
AACGCAG71150.032.1189846
CGCAGAG76200.030.0802888
ACGCAGA76400.029.8221637
AGAGTAC78650.029.05614311
CAGAGTA80200.028.49458110
GCAGAGT80650.028.2931089
AGTACGG48200.024.88222713
GAGTACT43500.024.80921212
GAGTACG48850.024.54681612
AGTACTT44050.024.1148213
GTACTTT48150.022.06629214
GTACGGG54950.021.8305314
Produced by FastQC (version 0.11.5)