FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860458
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACTGTTGGCAGTAATAAATTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC80500.9355482777776486No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35370.411060156335347No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC33920.3942086656176129No Hit
GTATAAAACTTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGGGAACTGTGGCTGCACCAT31980.37166253320905845No Hit
GTCCCTGATCTATGCTGCATCCATTTTGCAAAGTGGGGTCCCATCAAAGT31270.36341111361623696No Hit
GATCTATGCTGCATCCATTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA29550.3434217591096834No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG28960.33656494564522615No Hit
GCATAGATCAGGGACTGAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC26010.30228087832293965No Hit
GTATCAACGCAGAGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAATTGCAAAATCTTCAGGCTGCAGGCTG25340.2944943274395729No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24410.28368612994475034No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23200.2696238514837447No Hit
GATCAGGGACTGAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTAAAA22840.2654400330986521No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22010.25579400737746644No Hit
GTCTCCATCCTCACTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT21110.24533446141473494No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGAGGTGACAT21010.2441722896410981No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC20530.23859386512764133No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20520.23847764795027765No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT20380.2368506074671861No Hit
GTATCAACGCAGAGTACGGGAATCAGACCCAGTCAGGACACAGCATGGAC20210.23487491545200345No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT19860.23080731424427453No Hit
AGTCAGGGCATTAGCAATTTTTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTCAGTCCCTGATCTATG19400.22546132408554515No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG19200.2231369805382715No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18960.2203477682815431No Hit
GTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATTTTTTAGCCTGGTTTCAGCAGAAA18430.21418825788126786No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17050.19815028740507962No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAGGGAACTGTGGCTGCACCA16910.19652324692198808No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16900.1964070297446244No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC16640.1933853831331686No Hit
GTTTCCCAGGTGCCAGAGGTGACATCCAGATGACCCAGTCTCCATCCTCA16420.1908286052311676No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15900.18478531200825607No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC15750.18304205434780083No Hit
GGCCAAAAGTGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAATTGCA15300.17781228136643507No Hit
GTGCAGCCACAGTTCCCTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG15260.17734741265698037No Hit
TCCTCACTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCGGGC15090.17537172064179773No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14500.16851490717734044No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGAGGTGACATCCAGATGACCCAGTCTCCATCCTCA13560.15759049250515422No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13330.15491749742578953No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12950.15050124468596957No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12570.1460849919461496No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCCTTGATCTCCAGCTTGGTCC12290.14283091097996647No Hit
CCATCCTCACTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCG12060.14015791590060178No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12050.1400416987232381No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11970.13911196130432862No Hit
GTGCCAGAGGTGACATCCAGATGACCCAGTCTCCATCCTCACTGTCTGCATCTGTGGGAGACAGAGTCACCATCA11930.1386470925948739No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11370.13213893066250762No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11320.13155784477568924No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAATTTATTACTGCCAACAGTATAAAACTTAC11120.12923350122841556No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAATTTATTACTGCCAAC10890.12656050614905087No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10440.12133073316768511No Hit
GTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT10380.12063343010350301No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTG10300.11970369268459356No Hit
ATGCTGCATCCATTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA10160.118076652201502No Hit
GAATCAGACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTT10160.118076652201502No Hit
CATCCAGATGACCCAGTCTCCATCCTCACTGTCTGCATCTGTGGGAGACA10030.11656582889577412No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9960.11575230865422832No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9880.11482257123531887No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9840.11435770252586414No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9790.1137766166390457No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9780.11366039946168202No Hit
GGACCCCACTTTGCAAAATGGATGCAGCATAGATCAGGGACTGAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGG9600.11156849026913575No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTGATGGGACCCCA9530.11075497002758995No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9490.11029010131813521No Hit
GAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATTTTTTAGCCTGGTTTCAGCAGAAACCAGGGAAAG9490.11029010131813521No Hit
CTTGTCGGGCGAGTCAGGGCATTAGCAATTTTTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTCAGTCC9410.10936036389922577No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9350.10866306083504365No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9190.10680358599722474No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9180.10668736881986106No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9060.10529276269149684No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8960.10413059091786002No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8930.10378193938576896No Hit
ACCCAGTCTCCATCCTCACTGTCTGCATCTGTGGGAGACAGAGTCACCAT8890.10331707067631425No Hit
CACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATTTTTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCC8870.10308463632158688No Hit
GATCAGGGACTGAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTAAAAAATTGCTAATGCCCTGACTCGCCCG8750.10169003019322269No Hit
ATGCAGCATAGATCAGGGACTGAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTAAAAAATTGCTAATGCC8720.10134137866113163No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8680.1008765099516769No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8650.10052785841958584No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG200.007406944451.66400533
GTGGTAT4300.049.5114481
TGGTATC4100.049.4140552
GGTATCA14100.044.8106351
GTATCAA36050.040.1013681
TCTAGAC350.001379056339.2440953
TACCGCG350.001379844439.2395328
CTAGACT451.02939266E-438.1539844
TATCAAC39100.036.8854332
ATCAACG41850.034.5437133
TCAACGC41800.034.502884
CAACGCA43100.033.780885
AACGCAG44400.032.7917986
CGTATGC1600.032.4526941
TCGTATG1550.031.24245540
CGCAGAG46650.031.2065738
AGAGTGA22500.030.8985261
AATCTGT23500.030.55591268
AGAGTAC47900.030.46388611
ACGCAGA47950.030.3605167
Produced by FastQC (version 0.11.5)