FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860458
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACTGTTGGCAGTAATAAATTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC86171.001443417342857No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30730.35713538603859807No Hit
GTCCCTGATCTATGCTGCATCCATTTTGCAAAGTGGGGTCCCATCAAAGT29660.344700148060684No Hit
GTATAAAACTTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGGGAACTGTGGCTGCACCAT29640.34446771370595664No Hit
GTATCAACGCAGAGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAATTGCAAAATCTTCAGGCTGCAGGCTG28750.3341243849205888No Hit
GATCTATGCTGCATCCATTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA27380.3182026316217642No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC27250.3166918083160363No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG24540.2851969532504782No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22730.26416164414765164No Hit
GATCAGGGACTGAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTAAAA22310.2592805226983769No Hit
GCATAGATCAGGGACTGAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC21690.25207505770182853No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20140.23406139521045766No Hit
GTATCAACGCAGAGTACGGGAATCAGACCCAGTCAGGACACAGCATGGAC20070.23324787496891192No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19440.22592619279499987No Hit
GTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATTTTTTAGCCTGGTTTCAGCAGAAA19220.22336941489299886No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT19170.22278832900618045No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18550.21558286400963209No Hit
GTCTCCATCCTCACTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT18070.2100044394961753No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGAGGTGACAT17800.20686657570735587No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG17480.20314762603171801No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17380.20198545425808118No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC16930.19675568127671544No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAGGGAACTGTGGCTGCACCA16760.1947799892615328No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC16310.18955021628016708No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16010.18606370095925656No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15820.18385557458934662No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTGATGGGACCCCA15680.18222853410625506No Hit
AGTCAGGGCATTAGCAATTTTTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTCAGTCCCTGATCTATG15520.1803690592684361No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT15460.17967175620425402No Hit
GTGCAGCCACAGTTCCCTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14920.17339602862661513No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14570.1693284274188862No Hit
GGCCAAAAGTGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAATTGCA14560.16921221024152253No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14470.1681662556452494No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14430.16770138693579464No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14240.16549326056588468No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTG13710.15933375016560947No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGAGGTGACATCCAGATGACCCAGTCTCCATCCTCA13490.15677697226360846No Hit
GTCCCAGATCCACTGCCGCTGAACTTTGATGGGACCCCACTTTGCAAAAT13250.15398776000688005No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12900.14992015879915116No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC12800.14875798702551432No Hit
GTTTCCCAGGTGCCAGAGGTGACATCCAGATGACCCAGTCTCCATCCTCA12610.14654986065560435No Hit
GTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT12380.14387686557623963No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12360.14364443122151227No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11370.13213893066250762No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11150.1295821527605066No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCCTTGATCTCCAGCTTGGTCC11080.1287686325189608No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11070.12865241534159716No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10780.12528211719805035No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAATTTATTACTGCCAAC10640.12365507671495879No Hit
TCCTCACTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCGGGC10420.12109829881295775No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10270.1193550411525025No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAATTTATTACTGCCAACAGTATAAAACTTAC10180.11830908655622936No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10150.1179604350241383No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9950.11563609147686464No Hit
CCATCCTCACTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCG9790.1137766166390457No Hit
GTGCCAGAGGTGACATCCAGATGACCCAGTCTCCATCCTCACTGTCTGCATCTGTGGGAGACAGAGTCACCATCA9560.11110362155968101No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9380.10901171236713471No Hit
ATGCTGCATCCATTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA9330.1084306264803163No Hit
CTGCTGCTCTGTTTCCCAGGTGCCAGAGGTGACATCCAGATGACCCAGTC9060.10529276269149684No Hit
GAATCAGACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTT8930.10378193938576896No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8830.10261976761213214No Hit
GGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC8810.10238733325740478No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8800.1022711160800411No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8690.10099272712904059No Hit
GAGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAATTGCAAAATCTTC8630.10029542406485847No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)