FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1352519
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14940.11046055545245576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25150.042.241221
GTATCAA59850.039.522121
GTGGTAT8750.038.8994181
TGGTATC9600.035.0970652
TATCAAC71300.033.368182
TCAACGC72900.032.682984
ATCAACG73200.032.5490343
CAACGCA74300.032.2059675
AACGCAG76700.031.0189196
CTCGTAT1251.200533E-1030.45446639
ACGCAGA82550.028.8912037
CGCAGAG83000.028.7345648
TCGTATG1452.1827873E-1128.66510840
CGTATGC1352.910383E-1028.24379341
AGAGTAC86550.027.39714211
GCAGAGT87600.027.1864419
CAGAGTA89250.026.6453310
GAGTACG57150.023.63194812
AGTACGG57350.023.60945913
ACGGACT2155.456968E-1222.39577727
Produced by FastQC (version 0.11.5)