FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1352519
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18280.13515521778252282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9450.043.696651
GGTATCA27850.041.0215071
GTATCAA66650.039.4451
TGGTATC11100.035.933192
TATCAAC77050.034.049652
ATCAACG79900.032.728433
TCAACGC80300.032.3702624
CAACGCA81650.031.8594285
AACGCAG83600.031.0729476
CGCAGAG89200.029.1991218
ACGCAGA89650.029.0142777
AGAGTAC92500.028.23161711
CAGAGTA93200.028.01957710
GCAGAGT94750.027.5249949
GAGTACG62400.024.58191912
AGTACGG62200.024.49907313
TACGGGT13800.023.63361215
GAGTACT45650.023.52861612
CGAACTA2250.022.93564229
AGTACTT47800.022.33004413
Produced by FastQC (version 0.11.5)