FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840611
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34350.408631340774746No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24540.2919305124486832No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24230.288242718689144No Hit
GTCCCTGACAGGTTCAGTGGCAGAGGATCAGGCACAGATTTTACACTGAA21250.25279231416196074No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG21200.2521975087168738No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21180.25195958653883904No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21170.2518406254498216No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGGCTCCACAACTCCTGATGTATTTGGGTT20460.2433943881295867No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGGCTC20290.241372049616291No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCTCCGCCGAAAGTG20130.2394686721920127No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19820.23578087843247353No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19800.23554295625443875No Hit
CTACAAACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGACATCAAACG19110.2273346411122386No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT17570.20901463340356002No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17540.20865775013650784No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT17450.20758710033535133No Hit
GTAATAGACGGCCGTGTCCGCGGCAGTCACAGACCTCAGTTTTAGGGAGA17380.20675437271222957No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA17200.2046130731099165No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15760.18748267629141183No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCTCTGCCA15260.18153462184054217No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATACATCAGGAGTTGTGGAGCCTGCCCTGGCTTCTGCAGGTA14910.17737098372493343No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCC14890.17713306154689862No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13720.1632146141318636No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG13330.15857513166018528No Hit
GTATCTGGTGGCCCCATCAGTAGTGGTGGTTACTCCTGGAGTTGGATCCG13170.15667175423590698No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12820.15250811612029821No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTCTCACTTTCGGCGGAGGGA12470.14834447800468945No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12140.14441876206711546No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCTCTGCCA11730.13954135741740234No Hit
GTTCAGTGGCAGAGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG11730.13954135741740234No Hit
AGCCAGGGCAGGCTCCACAACTCCTGATGTATTTGGGTTCTAATCGGGCC11630.13835174652722843No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11560.13751901890410664No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11520.13704317454803708No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11470.1364483691029501No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11360.1351397971237588No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11210.1333553807884979No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11100.13204680880930655No Hit
CTCCACAACTCCTGATGTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT11060.131570964453237No Hit
GCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC10850.12907278158387173No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10850.12907278158387173No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGCTGACTCAGTCTCCCCTCTCCCTGTCCGTCACCC10800.12847797613878478No Hit
GTCTATTACTGTGCGAGAGTCGGCCGCAATTACGATTTTTGGAGAGGTTATCGGGACCCTAAATACTACAATTA10760.1280021317827152No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10760.1280021317827152No Hit
GTACCTGCAGAAGCCAGGGCAGGCTCCACAACTCCTGATGTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGA10630.1264556376254891No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG10620.12633667653647168No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10530.12526602673531514No Hit
CTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCC10510.12502810455728036No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10420.12395745475612381No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10320.12276784386594988No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGAGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCT10260.12205407733184553No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGCTGACTCAGTCTCCCCT10230.12169719406479335No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACT10220.12157823297577597No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC10040.11943693337346287No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGACATCAAACGAA10010.11908005010641069No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9800.11658186723704543No Hit
GCAGAAGCCAGGGCAGGCTCCACAACTCCTGATGTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT9760.11610602288097586No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGCTGACTCAGTCTCCCCTCTCC9740.11586810070294108No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9630.11455952872374976No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9580.11396472327866278No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGCTGA9570.11384576218964539No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9560.113726801100628No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACTCCTCTCACTTTCGGCGGAGGGA9550.1136078400116106No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9510.11313199565554104No Hit
AGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGGCTCCACAA9510.11313199565554104No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9370.11146654040929752No Hit
GTCTTATAGTGGGGACACCCACTACAACCCGTCCGTCAAGAGTCGAGCTA9320.11087173496421056No Hit
CCCCTCTCCCTGTCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG9250.11003900734108879No Hit
CTCCTGATGTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT9120.10849251318386269No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT9120.10849251318386269No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9090.10813562991681051No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9020.10730290229368875No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8940.10635121358154961No Hit
GGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGA8890.10575640813646264No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8740.10397199180120174No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8740.10397199180120174No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8660.1030203030890626No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAAC8610.10242549764397564No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8560.10183069219888866No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8490.10099796457576692No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8480.10087900348674952No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8480.10087900348674952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCTA756.8744885E-753.13950370
AACTGAT6650.050.94200570
GTGGTAT2900.049.8806341
TCGTATG1050.049.4284540
CGTATGC1050.046.16633641
GGTATCA10350.045.5890851
TGGTATC3300.043.8344962
ATCTCGT1050.042.78428337
GTATCAA27600.042.1782261
ACGAGAC1300.039.74715822
ATGCCGT1350.038.52495244
CTTATAC1450.038.004291
TATCAAC30600.037.930552
CGAGACG1300.037.09955623
ATCAACG31900.036.2768253
TCAACGC32500.035.6070984
TACACAT1850.035.372245
CGGGATA1950.035.318217
CAACGCA33300.034.751675
TATGCCG1400.034.6475543
Produced by FastQC (version 0.11.5)