FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121439
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11290.10067422302951833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47650.038.821051
GGTATCA20000.036.5503041
TATCAAC52850.035.066322
TGGTATC7200.033.8428732
ATCAACG56050.033.0643163
TCAACGC56200.032.9154
GTGGTAT7450.032.7072071
TCGTATG2550.032.5768540
CAACGCA57400.032.2866675
CGTATGC2700.032.0894141
AACGCAG59050.031.3263846
ACGAGAC3200.030.02021622
CTCGTAT2700.029.4519439
ACGCAGA63250.029.2874097
CGCAGAG64300.028.754528
AGAGTAC64850.028.5106511
CAGAGTA66800.027.72972910
GACGCTA756.3449115E-527.48260129
GCAGAGT67400.027.4319849
TCTCGTA2650.027.3687638
Produced by FastQC (version 0.11.5)