FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1986196
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42170.21231540089699102No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29880.15043832532136808No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29070.14636017794819847No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAGCATCCTCAGCCTCCACCC27730.13961361315801663No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27310.13749901822378052No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26880.13533407579111023No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25560.1286882059977968No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22190.11172109902547382No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21430.10789468914447517No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21290.10718982416639646No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20530.1033634142853978No Hit
GTACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA20400.1027088968057533No Hit
CCCTTGGACAGGCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA20380.10260820180888491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11500.046.34431
TGGTATC12800.041.615412
GGTATCA33000.040.532011
GTATCAA77700.039.606321
TATCAAC87900.034.7572442
ATCAACG92000.033.2620243
TCAACGC93000.032.919734
CAACGCA94500.032.4294325
AACGCAG96050.032.1553966
ACGCAGA104150.029.6545947
CGCAGAG105200.029.4890968
AGAGTAC104950.029.03543511
CAGAGTA107500.028.4431710
GCAGAGT109100.028.309139
ATCAGTG19950.025.65286370
AGTACGG76350.024.3181113
GAGTACT48950.024.25489412
GAGTACG77400.024.20625912
AGTACTT49000.023.81356413
ATAAACG1352.7796887E-722.8964183
Produced by FastQC (version 0.11.5)