FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717886
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG758.367351E-1041.5652340
GGTATCA11650.041.0279241
ACGAGAC1800.040.11783622
CTCGTAT801.5970727E-938.91551639
GTATCAA26800.038.1076621
GCGCTTA655.589591E-737.03184523
TAAGGCG1950.035.32256729
CGTATGC806.768278E-834.68150741
TAGGTCG400.002637890234.3867155
TATCAAC32150.031.8732242
TCTCGTA1101.036824E-931.42045238
CAACGCA32750.031.2892845
TCAACGC32650.031.2797974
ATCAACG32950.030.9950033
GCGAATC2150.030.46892433
AACGCAG34000.030.0378066
ATCTCCG2300.029.8931610
TATACAC2650.029.8450743
GTGGTAT3750.029.3433271
CCCACGA2250.029.03564819
Produced by FastQC (version 0.11.5)