FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717886
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13480.18777354621764458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33350.041.3645821
GGTATCA13700.037.9172171
GTGGTAT5700.035.6087951
TATCAAC39550.034.77132
TGGTATC5850.034.6739732
TCAACGC39450.034.5714764
ATCAACG39650.034.498163
CGCCGTA501.9061856E-434.3585625
CAACGCA40500.033.7505955
AACGCAG41650.032.9813276
CGCAGAG45350.030.6669548
ACGCAGA45100.030.5323857
AGAGTAC45550.030.45691511
GCTCGTT703.5950834E-529.86859348
CGGGTAC703.964078E-529.44403817
CAGAGTA47350.029.37162210
GCAGAGT47650.029.18679
CGACCGG850.00588634929.18304370
ACGGGTA1302.0736479E-1029.06655116
GAGTACG30400.026.20639412
Produced by FastQC (version 0.11.5)