FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences809303
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11800.14580447619741926No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGTTCAACTTCAGGGAAA11410.14098551469597914No Hit
GTTTTATACTGTTGGCAGTAATAAATTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC9150.1130602506107107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14150.042.840521
CACTACG350.0013602739.3534747
GTATCAA33050.037.3086931
GTGGTAT5250.036.738991
TAACGAA3750.036.54414770
TGGTATC5300.036.3923952
TATCAAC37300.032.8730242
TCAACGC38250.032.596854
ATCAACG38200.032.3690223
CAACGCA39250.031.7663565
AACGCAG39650.031.6196236
GGCGCTA450.00451760630.81004342
AGAGTAC43450.028.76611111
CGCAGAG43500.028.7330478
ACGCAGA44150.028.4677627
CAGAGTA44750.027.93044710
GCAGAGT45250.027.6218249
ACGCGTA500.00779880627.55254222
ACGTGGT1209.847998E-425.9546570
CTAACGA3800.025.07430669
Produced by FastQC (version 0.11.5)