FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences809303
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11020.136166553194539No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGTTCAACTTCAGGGAAA10790.13332460153984355No Hit
GTTTTATACTGTTGGCAGTAATAAATTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC10740.13270678596273583No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10070.12442805722949254No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8630.10663496860879053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5850.047.1140751
TGGTATC6000.043.0358772
TAACGAA4050.042.45852370
GGTATCA15800.040.121511
GTATCAA37950.038.0380971
TATCAAC42450.033.577112
ATCAACG44400.031.77853
TCAACGC44650.031.5693074
CAACGCA46000.030.9226515
AACGCAG46500.030.6621466
CTAACGA3750.030.09559869
CGCAGAG51000.027.8219328
AGAGTAC51800.027.7221611
CAGAGTA52200.027.5114310
ACGCAGA51900.027.3394687
GCAGAGT52700.027.1200269
AGGTACG658.760032E-426.4836182
GAGTACG34300.025.74224312
AGTACGG34400.025.5675413
TCTAACG4400.024.75800768
Produced by FastQC (version 0.11.5)