FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217993
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATG200.007452265451.5713617
GTATCAA10500.038.6287271
GTGGTAT1600.036.5213361
AACGCAG11500.035.269716
TATCAAC11700.035.2543832
AATAGCG400.00235227235.1883562
ATCAACG11650.035.1106383
CAACGCA11700.034.9605945
TCAACGC11950.034.2292024
ATAGCGG450.00414599931.34478663
ACGCAGA13100.030.9619587
TCACGTA450.004688008730.56782225
GGTATCA4050.030.5537991
TGCAAGC450.004698548530.5537972
CGCAGAG13400.030.268788
AGAGTAC13750.029.49830211
GCAGAGT13900.029.1799769
CAGAGTA13900.029.17997610
TGGTATC1950.028.2035052
CGTAGGA500.00782643327.52367228
Produced by FastQC (version 0.11.5)